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Immunologic properties of bacterial lipopolysaccharide (LPS). IV. Cellular basis of the unresponsiveness of C3H/HeJ mouse spleen cells to LPS-induced mitogenesis

Journal Article · · J. Immunol.; (United States)
OSTI ID:7317336
; ;
Lymphoid cells obtained from the C3H/HeJ mouse strain respond abnormally to lipopolysaccharides (LPS) in vitro, as shown by the fact that they are unable to make a mitogenic response to some LPS preparations and make only a low mitogenic response to other LPS preparations. In contrast, cells from a closely related C3H substrain, the C3H/St, are highly responsive to both types of LPS preparations. Experiments were carried out to determine the cellular basis of these genetically determined LPS response differences. This question was approached by studying the mitogenic response to LPS in cultures containing mixtures of various combinations of B cells, T cells, and macrophages from C3H/HeJ and C3H/St mice. The results obtained indicate that the defect in the C3H/HeJ mouse strain that limits its responsiveness to positive LPS and which renders it totally unresponsive to negative LPS appears to be an intrinsic defect in the capacity of B cells to react to the mitogenic stimulus of LPS.
Research Organization:
Scripps Clinic and Research Foundation, La Jolla, CA
OSTI ID:
7317336
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 118:1; ISSN JOIMA
Country of Publication:
United States
Language:
English

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Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CULTURES
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
KINETICS
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
LYMPHOCYTES
MAMMALS
MICE
MUTAGENESIS
ORGANIC COMPOUNDS
POLYSACCHARIDES
REACTION KINETICS
RODENTS
SACCHARIDES
SOMATIC CELLS
SPLEEN CELLS
VERTEBRATES