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Immunologic properties of bacterial lipopolysaccharide (LPS). II. The unresponsiveness of C3H/HeJ mouse spleen cells to LPS-induced mitogenesis is dependent on the method used to extract LPS

Journal Article · · J. Exp. Med.; (United States)
; ; ;

There exists a unique strain of mouse, the C3H/HeJ, that is inherently refractive to many of the diverse biological effects of bacterial lipopolysaccharide (LPS) which are characteristically displayed by LPS in other strains of mice. This defect in the C3H/HeJ appears to be specific to LPS, since other B-cell mitogens, such as purified protein derivative of tuberculin (PPD), dextran sulfate, and poly I sponsor quantitatively normal mitogenic responses in this strain. These observations have led to the concept that the C3H/HeJ mouse is a nonresponder to a number of LPS-induced biological phenomena. However, this report will present more recent evidence, with regard to the mitogenic activity of LPS, which demonstrates that the unresponsive state inherent in the C3H/HeJ mouse to LPS-induced mitogenesis is a condition which depends on the method used to extract LPS. These results will be considered within the mechanistic framework that activation of B cells by LPS is critically dependent on the structural integrity of both the LPS molecule and putative B-cell receptors for LPS.

Research Organization:
Scripps Clinic and Research Foundation, La Jolla, CA
OSTI ID:
6864904
Journal Information:
J. Exp. Med.; (United States), Journal Name: J. Exp. Med.; (United States) Vol. 142; ISSN JEMEA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CENTRIFUGATION
CENTRIFUGES
CHEMICAL ACTIVATION
COMPARATIVE EVALUATIONS
CONCENTRATORS
CONNECTIVE TISSUE CELLS
DNA REPLICATION
ESCHERICHIA COLI
EXTRACTION
IMMUNOLOGY
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MICROORGANISMS
MITOGENS
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
POLYSACCHARIDES
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
SPLEEN CELLS
ULTRACENTRIFUGES
VERTEBRATES