Stimulation of cAMP levels and modulation of antibody formation in mice immunized with cholera toxin. [Vibrio cholerae]

Journal Article · Fri Feb 28 00:00:00 EDT 1975 · Ann. N.Y. Acad. Sci.; (United States)

DOI:https://doi.org/10.1111/j.1749-6632.1975.tb29090.x· OSTI ID:7312200

Kateley, J R; Friedman, H

Injection of mice with 1.0 ..mu..g of a purified exotoxin derived from Vibrio cholerae together with a challenge injection of sheep erythrocytes (SRBC) or E. coli LPS markedly influenced the immune response to these antigens. Simultaneous injection of the toxin with antigen resulted in a delayed appearance of antibody-forming cells during the first few days after immunization, followed by a marked enhancement of the peak numbers of antibody-forming cells. In the case of the immune response to SRBC, both 19S and 7S plaque-forming cells (PFC) were enhanced on the peak day of response after simultaneous immunization of toxin-injected mice. The secondary immune response to SRBC was also similarly affected when cholera toxin was given along with a second injection of erythrocytes; i.e., a delay in appearance of the first antibody-forming cells followed by a marked enhancement of the peak 19S and 7S PFC response. Injection of cholera toxin 1-3 days prior to SRBC or LPS was immunosuppressive. The effect of cholera toxin on the level of splenic cyclic AMP appeared related to the effects on antibody formation.

• Research Organization: Albert Einstein Medical Center, Philadelphia
• OSTI ID: 7312200
• Journal Information: Ann. N.Y. Acad. Sci.; (United States), Journal Name: Ann. N.Y. Acad. Sci.; (United States) Vol. 249; ISSN ANYAA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
AMP
ANIMAL CELLS
ANIMALS
ANTIBODY FORMATION
ANTIGENS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHOLERA
CONNECTIVE TISSUE CELLS
DISEASES
IMMUNE REACTIONS
IMMUNITY
INFECTIOUS DISEASES
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MICE
MICROORGANISMS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
RODENTS
SOMATIC CELLS
THYMUS
TOXIC MATERIALS
TOXINS
VERTEBRATES