Fully automated solid-phase radioimmunoassay for triiodothyronine. [/sup 125/I tracer technique]
Journal Article
·
· Clin. Chem.; (United States)
OSTI ID:7310943
We report the first solid-phase radioimmunoassay for triiodothyronine with use of antibody-coated tubes, which is designed specifically for the fully automated radioimmunoassay instrument, Micromedic Systems' Concept 4 Automatic Radioassay. Antisera to triiodothyropropionic acid/bovine serum albumin were raised in rabbits, purified by ammonium sulfate precipitation, and coated onto polypropylene tubes. Analytical recovery of exogenous triiodothyronine added to sera from normal men and women and pregnant women was quantitative. Intra-assay and inter-assay coefficients of variation are 4 to 5 and 6 to 9 percent, respectively. Correlation coefficients (r) for comparison of sample values with those obtained by three commercial laboratories were 0.95, 0.84, and 0.91. The sensitivity of the assay is 0.5 ..mu..g/liter. The assay can be performed either manually or be fully automated on the Concept 4.
- Research Organization:
- Micromedic Systems, Fort Collins, CO
- OSTI ID:
- 7310943
- Journal Information:
- Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 23:5; ISSN CLCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AUTOMATION
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
PEPTIDE HORMONES
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
THYROID HORMONES
TRACER TECHNIQUES
TRIIODOTHYRONINE
VERTEBRATES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AUTOMATION
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
PEPTIDE HORMONES
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
THYROID HORMONES
TRACER TECHNIQUES
TRIIODOTHYRONINE
VERTEBRATES