Ethidium bromide induced cytoplasmic male sterility in pearl millet

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Crop Sci.; (United States)

DOI:https://doi.org/10.2135/cropsci1976.0011183X001600050035x· OSTI ID:7308446

Burton, G W; Hanna, W W

Seeds of homozygous ''Tift 23DB/sub 1/'' pearl millet (Pennisetum americanum (L.) K. Schum), maintainer for cytoplasmic male sterile (cms) ''Tift 23DA/sub 1/,'' were soaked in deionized water solutions of 250 and 1000 ppM of ethidium bromide (EB) at 5/sup 0/C for 40 hours. Treated seeds were rinsed in tap water for 30 min, surface-dried, and planted in replicated two-row plots in the field with two-row plots of untreated Tift 23DB/sub 1/. Male-sterile mutants (whole heads, sectored heads, or heads with intermingled male-sterile and male-fertile florets) occurred at frequencies of 1 in 30,100, 1 in 515, and 1 in 274 in total head populations of 30,100, 22,700, and 19,200 treated with 0, 250, and 1000 ppM EB, respectively. Occurrence of M/sub 1/ sterile mutants that set seed when pollinated and gave rise to some cms progenies in the greenhouse proved that the mutants were cytoplasmic male sterile.

• Research Organization: Univ. of Georgia, Tifton
• OSTI ID: 7308446
• Journal Information: Crop Sci.; (United States), Journal Name: Crop Sci.; (United States) Vol. 16:5; ISSN CRPSA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
553000 -- Agriculture & Food Technology
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
AQUEOUS SOLUTIONS
BIOLOGICAL EFFECTS
BIOMASS
CELL CONSTITUENTS
CEREALS
CYTOPLASM
DISPERSIONS
ENERGY SOURCES
GENETIC EFFECTS
GRAMINEAE
GRASS
HYBRIDIZATION
MALES
MILLET
MIXTURES
MUTAGENS
MUTANTS
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PLANTS
PROGENY
RENEWABLE ENERGY SOURCES
REPRODUCTION
SEEDS
SOLUTIONS
STERILITY