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Stable cytoplasmic male-sterile mutants induced in Tift 23DB/sub 1/ pearl millet with mitomycin and streptomycin

Journal Article · · Crop Sci.; (United States)
;
Stable cytoplasmic male-sterile lines are necessary for the commercial production of F/sub 1/ hybrid seed of important crop plants such as sorghum (Sorghum bicolor L.) and pearl millet (Pennisetum americanum (L.) Leeke.). The objective of this investigation was to ascertain if streptomycin and mitomycin could help to create stable cms mutants. Seeds of Tift 23DB/sub 1/ pearl millet were soaked in water solutions of 200 and 500 ppM of streptomycin, and 50 ppM of mitomycin at 5 C for 40 hours. Treated seeds were rinsed in tap water, surface dried, and planted in an isolated field along with a check of untreated seed. Head populations of 32,800, 22,300, 18,000, and 13,000 from seed treated with 0, 200, 500 ppM of streptomycin and 50 ppM of mitomycin contained progeny tested stable cytoplasmic male-sterile (cms) mutants at frequencies of 1 in 16,200, 5575, 4500, and 2600, respectively. The sterility or fertility of cms mutants crossed with 2 sterility maintainers and 6 fertility restorer inbreds of pearl millet suggests that the induced mutants have sterility maintainer and fertility restorer requirements similar to cms Tift 23DA/sub 1/ and are similar cms mutants.
Research Organization:
Univ. of Georgia, Tifton
DOE Contract Number:
AS09-76EV00637
OSTI ID:
6437715
Journal Information:
Crop Sci.; (United States), Journal Name: Crop Sci.; (United States) Vol. 22; ISSN CRPSA
Country of Publication:
United States
Language:
English

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Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CEREALS
CYTOPLASM
DATA
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EXPERIMENTAL DATA
FERTILITY
GENETIC EFFECTS
GRAMINEAE
GRASS
INFORMATION
MILLET
MITOMYCIN
MUTAGENESIS
MUTANTS
NUMERICAL DATA
PLANTS
SEEDS
STERILITY
STREPTOMYCIN