Organ culture-cell culture system for studying multistage carcinogenesis in respiratory epithelium. [Mice]

Conference · Sat Jan 01 04:00:00 EST 1977

OSTI ID:7304800

Steele, Vernon E.; Marchok, Ann C.; Nettesheim, Paul

An organ culture-cell culture system was used to demonstrate carcinogen dose-dependent transformation of tracheal epithelial cells in vitro. Tracheal explants were exposed to MNNG (N-methyl-N/sup 1/-nitro-N-nitrosoguanidine) in organ culture. Outgrowths from these explants provided epithelial cell cultures. The numbers of long term epithelial cell cultures and cell lines that were established per explant increased as MNNG exposure concentration increased. At the present time, more cell lines derived from explants exposed to the highest MNNG concentration have produced palpable tumors than cell lines derived from explants exposed to lower MNNG concentrations. No cell lines were established from primaries derived from control explants. TPA (12-0-tetradecanoyl-phorbol-13-acetate), stimulates DNA synthesis in tracheal epithelium in organ culture in a manner simular to that described for mouse skin. Short exposures to TPA not only stimulated DNA synthesis earlier, but the stimulation was greater than that obtained with continuous exposure. At the present time, exposure of tracheal organ cultures to MNNG followed by TPA has resulted in an enhanced production of morphologically altered cells in primary epithelial cell cultures, than exposure to either agent alone.

• Research Organization: Oak Ridge National Lab., TN (USA)
• Sponsoring Organization: US Energy Research and Development Administration (ERDA)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 7304800
• Report Number(s): CONF-770342--2
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARCINOGENESIS
CARCINOGENS
CELL CULTURES
DISEASES
DNA
EPITHELIUM
MAMMALS
MICE
MORPHOLOGICAL CHANGES
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
RESPIRATORY SYSTEM
RODENTS
SYNTHESIS
TISSUES
TRACHEA
VERTEBRATES