Modification of glycerolipid metabolism in L-M fibroblasts by an unnatural amino-alcohol, N-isopropylethanolamine
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
The unnatural amino-alcohol, N-isopropylethanolamine, is incorporated into a phospholipid by monolayers of L-M fibroblasts. This phospholipid was identified as 1, 2-diacyl-sn-glycero-3-phosphoisopropylethanolamine by using chemical and enzymatic procedures combined with thin-layer and gas-liquid chromatography. Since the phospho-N-isopropylethanolamine moiety is removed by phospholipase C, the stereochemistry of the phospholipid analog is identical to naturally occurring phosphoglycerides. Incubation of cells in 10 mM N-isopropylethanolamine inhibited the incorporation of (/sup 14/C)choline and /sup 14/C)ethanolamine into phospholipids and stimulated the incorporation of (1-/sup 14/C)palmitic acid and (1-/sup 14/C)hexadecanol into triacylglycerols and alkyldiacylglycerols. These results indicate that N-isopropylethanolamine affects glycerolipid synthesis at the diradylglycerol branch point. (auth)
- Research Organization:
- Oak Ridge Associated Universities, TN
- OSTI ID:
- 7296046
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 62:4; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Regulation of glycerolipid metabolism in Tetrahymena species
Sphingosine and phorbol ester preferentially stimulate phosphatidylethanolamine hydrolysis
Involvement of triacylglycerol in the metabolism of fatty acids by cultured neuroblastoma and glioma cells
Thesis/Dissertation
·
Thu Dec 31 23:00:00 EST 1987
·
OSTI ID:6948170
Sphingosine and phorbol ester preferentially stimulate phosphatidylethanolamine hydrolysis
Conference
·
Sun Mar 10 23:00:00 EST 1991
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
·
OSTI ID:5264107
Involvement of triacylglycerol in the metabolism of fatty acids by cultured neuroblastoma and glioma cells
Journal Article
·
Tue Nov 30 23:00:00 EST 1982
· J. Lipid Res.; (United States)
·
OSTI ID:5821084
Related Subjects
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON 14
CARBON ISOTOPES
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
ESTERS
EVEN-EVEN NUCLEI
FIBROBLASTS
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MEMBRANES
METABOLISM
MOLECULAR BIOLOGY
NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
RADIOISOTOPES
SEPARATION PROCESSES
SOMATIC CELLS
TISSUE CULTURES
YEARS LIVING RADIOISOTOPES
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON 14
CARBON ISOTOPES
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
ESTERS
EVEN-EVEN NUCLEI
FIBROBLASTS
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MEMBRANES
METABOLISM
MOLECULAR BIOLOGY
NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
RADIOISOTOPES
SEPARATION PROCESSES
SOMATIC CELLS
TISSUE CULTURES
YEARS LIVING RADIOISOTOPES