Sphingosine and phorbol ester preferentially stimulate phosphatidylethanolamine hydrolysis
- Univ. of Minnesota, Austin (United States)
It is generally accepted that agonist-stimulated phosphoinositide-specific phospholipase C and phosphatidyl-choline (PrdCho)-specific phospholipase D are the major systems to produce the lipid messengers phosphatidic acid (PtdOH) and 1,2-diacylglycerol (DAG). Here the authors show that simultaneous treatment of ({sup 14}C)palmitate-prelabeled NIH 3T3 fibroblasts with two synergistically acting mitogens, sphingosine and 12-O-tetradecanoylphorbol 13-acetate (TPA), resulted in about a two-fold increase in the cellular level of PtdOH, and that both sphingosine, and to a lesser extent, TPA preferentially stimulated phosphatidyl-ethanolamine (PtdEtn) hydrolysis. This latter point was demonstrated by using NIG 3T3 cells prelabeled with {sup 14}C-labeled bases, {sup 32}P-labeled phospholipids or ({sup 14}C)palmitate. Treatment of ({sup 14}C)palmitate-prelabeled cells with TPA alone did not result in significant accumulation of PtdOH, due to rapid metabolism of this phospholipid. On the other hand, sphingosine inhibited the rapid metabolism of the PtdOH pool, formed through the action of phospholipase D, by inhibiting PtdCho synthesis. Since PtdOH is a potent mitogen in these cells, it is possible that these effects of sphingosine on PtdOH metabolism are related to its recently reported co-mitogenic effects.
- OSTI ID:
- 5264107
- Report Number(s):
- CONF-9104107--
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:4; ISSN FAJOE; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARCINOGENS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
FIBROBLASTS
HEXADECANOIC ACID
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPASES
LIPIDS
METABOLISM
MITOGENS
MONOCARBOXYLIC ACIDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHORBOL ESTERS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
SPHINGOMYELINS
SYNERGISM
TRACER TECHNIQUES