Homing of bone marrow lymphoid cells

Journal Article · Mon May 01 04:00:00 EDT 1978 · Transplantation; (United States)

DOI:https://doi.org/10.1097/00007890-197805000-00005· OSTI ID:7286952

Yoshida, Y; Osmond, D G

DNA labeling, bone marrow fractionation, and radioautography were used to follow the fate of transfused, newly formed marrow lymphocytes in irradiated hosts. After infusing donor Hartley guinea pigs with /sup 3/H-thymidine for 3 to 5 days, high concentrations of labeled small lymphocytes and large lymphoid cells were separated from marrow by sedimentation in sucrose-serum gradients and injected into lethally x-irradiated syngeneic recipients. Most labeled small lymphocytes and large lymphoid cells rapidly left the circulation. They appeared to be mainly in the marrow and spleen, increasing in incidence from 1 to 3 days, but declining in mean grain count. Labeled cells were scattered throughout the recipient marrow; in the spleen they localized initially in the red pulp, and subsequently in peripheral areas of white pulp, often in clusters. Labeled small lymphocytes showed a delayed migration into the mesenteric lymph node, mainly in the superficial cortex and medulla; they also appeared in small numbers in Peyer's patches, but rarely in the thymus or thoracic duct lymph. It is concluded that a rapid selective homing of newly formed marrow lymphoid cells occurs in both the marrow and certain areas of the spleen of irradiated hosts, followed by a continuing proliferation of large lymphoid cells and production of small lymphocytes. The results are discussed with respect to the life history of marrow lymphocytes and the use of adoptive immune assays of marrow cells to characterize B lymphocyte maturation.

• Research Organization: McGill Univ., Montreal
• OSTI ID: 7286952
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 25:5; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
BONE MARROW CELLS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DISTRIBUTION
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
GUINEA PIGS
HEMATOPOIETIC SYSTEM
HETEROCYCLIC COMPOUNDS
IMMUNE REACTIONS
IMMUNOSUPPRESSION
IONIZING RADIATIONS
IRRADIATION
LABELLED COMPOUNDS
LETHAL IRRADIATION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RIBOSIDES
RODENTS
SOMATIC CELLS
SPLEEN
THYMIDINE
TISSUE DISTRIBUTION
TISSUES
TRANSPLANTS
TRITIUM COMPOUNDS
VERTEBRATES
WHOLE-BODY IRRADIATION
X RADIATION