Radiation-induced alterations in murine lymphocyte homing patterns. I. Radiolabeling studies. [X Radiation, /sup 51/Cr tracer technique]

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(76)90162-3· OSTI ID:7086775

Crouse, D A; Feldbush, T L; Evans, T C

In vitro x-irradiation of /sup 51/Cr-labeled spleen, lymph node, bone marrow, or thymus cells was found to alter their subsequent in vivo distribution significantly in syngeneic BDF/sub 1/ mice. Irradiated cells demonstrated an increased distribution to the liver and a significantly lower retention in the lungs. Cells going to the lymph nodes or Peyer's patches showed a significant exposure-dependent decrease in homing following irradiation. Irradiated lymph node cells homed in greater numbers to the spleen and bone marrow, while irradiated cells from other sources showed no preferential distribution to the same tissues. Sampling host tissues at various times after irradiation and injection did not demonstrate any return to normal patterns of distribution. The alterations in lymphocyte homing observed after in vitro irradiation appear to be due to the elimination of a selective population of lymphocytes or membrane alterations of viable cells, and the detection of these homing changes is in turn dependent upon the relative numbers of various lymphoid subpopulations which are obtained from different cell sources. Radiation-induced alterations in the normal homing patterns of lymphoid cells may thus be of considerable importance in the evaluation of subsequent functional assays in recipient animals.

• Research Organization: Univ. of Iowa, Iowa City
• OSTI ID: 7086775
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 27; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DISTRIBUTION
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HEMATOPOIETIC SYSTEM
IN VITRO
INTERMEDIATE MASS NUCLEI
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LUNGS
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MICE
NUCLEI
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
SPLEEN
TISSUES
TRACER TECHNIQUES
VERTEBRATES
X RADIATION