Factors influencing the survival of rat brain tumor cells after in vitro treatment with 1,3-bis(2-chloroethyl)-1-nitrosourea

Journal Article · Sun Jun 01 04:00:00 EDT 1975 · Cancer Res.; (United States)

OSTI ID:7286119

Wheeler, K T; Tel, N; Williams, M E; Sheppard, S; Levin, V A; Kara, P M

The shape of dose-response curves obtained for asynchronous, exponential growing 9L rat brain tumor cells treated in vitro with 1,3-bis(2-chloroethyl)-l-nitrosourea changed as a function of the drug exposure time. For short treatment times (<1 hr), the dose-response curves had shoulders, indicating that the cells may accumulate sublethal damage; however, after longer treatments (>1 hr), little if any shoulder was apparent. The slope of the exponential portion of the dose-response curve increased progressively with treatment periods from 15 min to 2 hr. Longer exposure times (up to 24 hr) produced no further changes in the cell-kill kinetics. Cell survival was directly related to the BCNU exposure dose and to the amount of bound BCNU per cell. Extrapolation of the curves for these two variables indicated that some BCNU damage accumulates before death occurs. The amount of serum and cell products available in the medium to bind BCNU affected the level of survival; however, there was no evidence that extracellular spontaneous breakdown products or chemical transformation products were involved in the cell-killing mechanism. (auth)

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 7286119
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 35; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
AMIDES
ANIMALS
BIOLOGICAL EFFECTS
BODY
BRAIN
CARBONIC ACID DERIVATIVES
CENTRAL NERVOUS SYSTEM
CHEMOTHERAPY
DISEASES
DOSE-RESPONSE RELATIONSHIPS
IN VITRO
MAMMALS
NEOPLASMS
NERVOUS SYSTEM
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RATS
RODENTS
SURVIVAL CURVES
THERAPY
TISSUE CULTURES
UREA
VERTEBRATES