Factors influencing the survival of rat brain tumor cells after in vitro treatment with 1,3-bis(2-chloroethyl)-1-nitrosourea

Journal Article · Sun Jun 01 00:00:00 EDT 1975 · Cancer Res.; (United States)

OSTI ID:7286119

Wheeler, K T; Tel, N; Williams, M E; Sheppard, S; Levin, V A; Kara, P M

The shape of dose-response curves obtained for asynchronous, exponential growing 9L rat brain tumor cells treated in vitro with 1,3-bis(2-chloroethyl)-l-nitrosourea changed as a function of the drug exposure time. For short treatment times (<1 hr), the dose-response curves had shoulders, indicating that the cells may accumulate sublethal damage; however, after longer treatments (>1 hr), little if any shoulder was apparent. The slope of the exponential portion of the dose-response curve increased progressively with treatment periods from 15 min to 2 hr. Longer exposure times (up to 24 hr) produced no further changes in the cell-kill kinetics. Cell survival was directly related to the BCNU exposure dose and to the amount of bound BCNU per cell. Extrapolation of the curves for these two variables indicated that some BCNU damage accumulates before death occurs. The amount of serum and cell products available in the medium to bind BCNU affected the level of survival; however, there was no evidence that extracellular spontaneous breakdown products or chemical transformation products were involved in the cell-killing mechanism. (auth)

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 7286119
• Journal Information: Cancer Res.; (United States), Vol. 35
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NEOPLASMS
CHEMOTHERAPY
NITROSO COMPOUNDS
DOSE-RESPONSE RELATIONSHIPS
BIOLOGICAL EFFECTS
BRAIN
IN VITRO
RATS
SURVIVAL CURVES
TISSUE CULTURES
UREA
AMIDES
ANIMALS
BODY
CARBONIC ACID DERIVATIVES
CENTRAL NERVOUS SYSTEM
DISEASES
MAMMALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RODENTS
THERAPY
VERTEBRATES
550900* - Pathology