Strings of contiguous modified pentanucleotides with increased DNA-binding affinity can be used for DNA sequencing by primer walking
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow (Russian Federation)
Modified oligonucleotides containing 5-methylcytidine and/or 2-aminoadenosine form tighter hybrids with DNA and are, therefore, more efficient primers for DNA sequencing as compared to their natural counterparts. Strings of contiguous modified pentanucleotides can be used for DNA sequencing by primer walking.
- OSTI ID:
- 7285396
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 90:22; ISSN PNASA6; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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