A subset of 1200 hexamers is sufficient to sequence over 95% of cDNAs by hexamer string primer walking
Journal Article
·
· Genomics; (United States)
- Lawrence Livermore National Lab., CA (United States)
It has recently been discovered that DNA sequencing reactions can be successfully primed by contiguous strings of three or more hexamer oligonucleotides. Thus, with the complete set of 4096 hexamers it should now be feasible to sequence DNA templates by primer walking. This method may work best for cDNAs, which have both shorter templates and less repetitive sequence than commonly sequenced genomic templates such as cosmids. To investigate streamlining the primer walking procedure, the authors explored the possibility of using a subset of hexamers. 5 refs., 1 fig.
- OSTI ID:
- 6975712
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 17:3; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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