SP-Sephadex equilibrium chromatography of bradykinin and related peptides: application to trypsin-treated human plasma
An analytical method is described for the separation of bradykinin. Lysbradykinin, and Met-Lys-bradykinin by equilibrium chromatography on SP-Sephadex C-25 eluted in 0.02 M Tris-HCl buffer, pH 8.10, 0.12 M NaCl. A second elution buffer, 0.02 M Tris-HCl buffer, pH 7.70, 0.06 M NaCl, serves as a second parameter for the identification of bradykinin and also separates the hormone from plasma bradykinin-potentiating peptides. Ten to one-hundred nanomoles of each peptide can be recovered in high yields, identified by elution position, and measured by bioassay with the isolated guinea pig ileum. The identification of bradykinin as the peptide released by trypsin acting on acid-denatured plasma is documented as an illustration of the method.
- Research Organization:
- Brookhaven National Lab., Upton, NY
- OSTI ID:
- 7285265
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 81:2; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400105 -- Separation Procedures
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
BRADYKININ
CHEMICAL ANALYSIS
CHROMATOGRAPHY
ENZYMES
HYDROLASES
KININS
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PEPTIDES
PROTEINS
SEPARATION PROCESSES
TRYPSIN