Solute concentration affects bradykinin-mediated increases in renal prostaglandin E2
The effects of solute concentration on the bradykinin-mediated increase in inner medullary slice prostaglandin E2 (PGE2) synthesis were investigated. PG content was determined by specific RIA. Bradykinin stimulation was prevented by the addition of the following solutes to Krebs buffer: 1.0 M urea, 0.5 or 1.0 M NaCl, 0.5 or 1.0 M mannitol, 1.0 M urea plus 0.5 M NaCl, or 1.0 M mannitol plus 0.5 M NaCl. By contrast, basal PGE2 synthesis was increased by 1.0 M mannitol or by 1.0 M mannitol plus 0.5 M NaCl, but decreased by 1.0 M urea. Urea elicited a concentration-dependent, reversible inhibition of bradykinin stimulation, with 0.01 M urea being the lowest effective concentration. By contrast, basal PGE2 synthesis was only reduced at a urea concentration greater than 0.6 M. Arachidonic acid-mediated increases in both PGE2 and PGF2 alpha synthesis were not prevented by 1.0 M urea. The latter suggests that neither PG endoperoxide synthetase nor PG endoperoxide E isomerase are inhibited by urea. The data indicate that different hypertonic solutions have different effects on basal PG production, but all inhibit bradykinin stimulation.
- Research Organization:
- Geriatric Research, Education, and Clinical Center, Veterans Administration Medical Center, St. Louis, Missouri
- OSTI ID:
- 5346729
- Journal Information:
- Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 109:6; ISSN ENDOA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
AMIDES
ARACHIDONIC ACID
BIOSYNTHESIS
BODY
BRADYKININ
BUFFERS
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CHLORIDES
CHLORINE COMPOUNDS
ENZYME ACTIVITY
ENZYMES
HALIDES
HALOGEN COMPOUNDS
ISOMERASES
ISOTOPE APPLICATIONS
KIDNEYS
KININS
LIGASES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDES
PROSTAGLANDINS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
SODIUM CHLORIDES
SODIUM COMPOUNDS
SYNTHESIS
TRACER TECHNIQUES
UREA