Ozonation of DNA forms adducts: A [sup 32]P-DNA labeling and thin-layer chromatography technique to measure DNA environmental biomarkers

Journal Article · · Archives of Environmental Health; (United States)

DOI:https://doi.org/10.1080/00039896.1994.9934411· OSTI ID:7284329

Cajigas, A; Steinberg, J J ^[1]; Gayer, M ^[2]; Beam, C ^[3]

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1. Albert Einstein College of Medicine, New York, NY (United States)
2. Columbia Univ., New York, NY (United States)
3. City Univ. of New York, NY (United States)

Little direct documented evidence of ozone's genotoxicity exists. Deoxyribonucleic acid (DNA) adducts are produced by environmental toxic agents, including ozone. We have described a modified thin-layer chromatography (TLC) technique that can assess adduct formation as a biomarker of ozone injury. This requires [sup 32]P-labeling DNA, digestion of deoxynucleotides (dNMPs), and separation in two-dimensional PEI-cellulose TLC. We have applied this technique to control DNAs, to control DNA in solution exposed to acute ambient ozone, and to control DNA exposed to acute bubbled-through ozone (2 ppm for 24 h). We detected stable DNA adducts, including hydroxymethyluracil (HMU), thymine glycol (TG), 8-hydroxyguanine (8-OHG), and demonstrated, as yet, unidentified adducts that may serve as a [open quotes]fingerprint[close quotes] pattern of DNA adduction. This technique quantifies low-molecular-mass DNA adducts, both in vivo and in vitro, with potential applications to environmental toxicology. 89 refs., 6 figs., 2 tabs.

• OSTI ID: 7284329
• Journal Information: Archives of Environmental Health; (United States), Journal Name: Archives of Environmental Health; (United States) Vol. 49:1; ISSN 0003-9896; ISSN AEHLAU
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
BIOLOGICAL MARKERS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DETECTION
DNA
DNA ADDUCTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OZONE
OZONIZATION
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TOXICITY