Multiple DNA adducts in lymphocytes of smokers and nonsmokers determined by sup 32 P-postlabeling analysis

Technical Report · Mon Jan 01 04:00:00 EST 1990

OSTI ID:6140924

Jahnke, G D; Thompson, C L; Walker, M P; Gallagher, J E; Lucier, G W

Identification of DNA adducts in peripheral lymphocytes could serve as a means of monitoring human exposure to potential genotoxic agents. In the study, DNA from peripheral lymphocytes of smokers and nonsmokers was examined for adducts by the P1 nuclease {sup 32}P-post-labeling technique. Thin layer chromatography (TLC) maps from both groups revealed multiple DNA adducts which ranged from no adducts for one individual to six adducts for a different individual. The total DNA adduct concentrations were approximately one adduct in 10 to the seventh-10 to the eighth power normal nucleotides. Comparison of the adduct TLC profiles revealed individual variation in both pattern and level of DNA adducts. The type and amount of adduct was not influenced by smoking history and remained unchanged in four out of six subjects who were resampled after a one month interval. One adduct detected in lymphocyte DNA co-migrated on TLC with an adduct derived by in vitro incubation of lymphocytes with benzo(a)pyrene (B(a)P). The 3H-nucloside values were consistent with values obtained by {sup 32}P-postlabeling of the same sample (correlation coefficient of 0.88). No relationship was apparent between the capacity of lymphocytes to form a (3H)-B(a)P-derived adduct in vitro and the concentration of the adduct, or total adducts present in untreated lymphocytes.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
• OSTI ID: 6140924
• Report Number(s): PB-91-116897/XAB; EPA--600/J-90/177
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AEROSOLS
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMATOGRAPHY
COLLOIDS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISPERSIONS
DNA ADDUCTS
GENETIC EFFECTS
HYDROCARBONS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
METABOLISM
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RESIDUES
SEPARATION PROCESSES
SMOKES
SOLS
SOMATIC CELLS
SYNTHESIS
THIN-LAYER CHROMATOGRAPHY
TOBACCO SMOKES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES