Inactivation of poliovirus in wastewater sludge with radiation and thermoradiation

Journal Article · Sun May 01 04:00:00 EDT 1977 · Appl. Environ. Microbiol.; (United States)

OSTI ID:7282058

Ward, R L

The effect of sludge on the rate of viral inactivation by radiation and thermoradiation was determined. The virus used for the experiments was the poliovirus type 1 strain CHAT, which was grown in HeLa cells. Radiation, heat, and thermoradiation treatments were carried out in a chamber specifically designed to permit rapid heating and cooling of the samples at the beginning and completion of treatment, respectively. The treated samples were then assayed for plaque-forming units on HeLa cells after sonication in 0.1% sodium dodecylsulfate (SDS). For the radiation treatment virus was diluted 10-fold into PBS containing new sludge, irradiated at 20/sup 0/C with /sup 137/Cs at a dose rate of 30 krads/min, and assayed for infectious virus. The results show that raw sludge is protective of poliovirus against ionizing radiation but that small concentrations of sludge are nearly as protective as large concentrations. When heat and radiation are given simultaneously, however, the amount of protection afforded by sludge is less than the additive effects of the individual treatments. This result is especially evident at low concentrations of sludge. It appears, therefore, that thermoradiation treatment may be an effective way of inactivation viruses in waters containing low concentrations of suspended solids. (FMM)

• Research Organization: Sandia Labs., Albuquerque, NM
• OSTI ID: 7282058
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 33:5; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550700 -- Microbiology
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
560202* -- Thermal Effects-- Microorganisms-- (1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CESIUM 137
CESIUM ISOTOPES
ENERGY
HEAT
HELA CELLS
HYDROGEN COMPOUNDS
INACTIVATION
IONIZING RADIATIONS
IRRADIATION
ISOTOPES
LIQUID WASTES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
OXYGEN COMPOUNDS
PARASITES
POLIO VIRUS
RADIATION PROTECTION
RADIATIONS
RADIOISOTOPES
RESPONSE MODIFYING FACTORS
SLUDGES
VIRUSES
WASTE WATER
WASTES
WATER
YEARS LIVING RADIOISOTOPES