Technical progress report, August 1, 1975--July 31, 1976. [Biochemical studies on RNA of tobacco mosaic virus]

Technical Report · Thu Jan 01 04:00:00 EST 1976

DOI:https://doi.org/10.2172/7279483· OSTI ID:7279483

Siegel, A.

Previous work had demonstrated the presence of a unique low-molecular-weight RNA component (LMC) in extracts of tobacco mosaic virus (TMV) infected tissue. Enough of this component has been isolated during the past year to ascertain that it has a molecular weight of 250,000 daltons and that it acts as an in vitro messenger for the synthesis of TMV capsid protein. Thus, we conclude that at least one monocistronic messenger RNA for a virion coded product is generated during TMV infection. Strains of TMV were classified according to nucleotide sequence homology of their RNAs. The strains fall into groups by the test employed. No differences were observed between strains within a group, whereas no homology was detected between groups. Using this information, it was possible, in part, to relate differences in capsid protein amino acid sequences to the degree of nomology of their nucleotide coding sequences. A study was initiated into the Pot Y virus group infection mechanism. In contrast to TMV infection, it was determined that for both tobacco etch and potato virus Y that: viral RNA synthesis is inhibited by actinomycin B and synthesis by virus-related proteins is inhibited by chloramphenicol.

• Research Organization: Wayne State Univ., Detroit, Mich. (USA)
• Sponsoring Organization: USDOE
• OSTI ID: 7279483
• Report Number(s): COO-2384-5
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACTINOMYCIN
ANTIBIOTICS
ANTIMITOTIC DRUGS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CHEMISTRY
CHLORAMPHENICOL
DRUGS
INFECTIVITY
MICROORGANISMS
MOLECULAR WEIGHT
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RNA
SYNTHESIS
TOBACCO MOSAIC VIRUS
VIRUSES