Effect of tobacco mosaic virus infection on host and virus-specific protein synthesis in protoplasts

Journal Article · Sat Apr 01 04:00:00 EST 1978 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(78)90456-7· OSTI ID:7075927

Siegel, A; Hari, V; Kolacz, K

The nature and rate of virus-specific protein synthesis were determined in tobacco mosaic virus-infected protoplasts as a function of time after inoculation. Samples of infected and mock-infected protoplasts were exposed to radioactive amino acid for relatively short sequential time periods and the consequent labeled proteins were assessed following SDS-polyacrylamide gel electrophoresis and fluorography. The synthesis of three virus-specific proteins of molecular weights 160,000, 135,000, and 17,500 was confirmed. Synthesis of all three proteins was first detected during the 5- to 7-hr postinoculation period at which time the synthetic rate of the 135,000-dalton protein was greatest.This was soon overtaken by the 17,500-dalton capsid protein, the synthetic rate of which kept increasing until it accounted for a major portion of total protoplast protein synthesis. At 1 day postinoculation, it accounted for 50% and, at not quite 2 days, 70% of the total protein synthesis. Evidence is presented to suggest that virus-specific protein synthesis occurs in addition to, rather than at the expense of, normal cellular protein synthesis.

• Research Organization: Wayne State Univ., Detroit
• OSTI ID: 7075927
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 85:2; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
553001 -- Agriculture & Food Technology-- Tracer Techniques-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
AMINO ACIDS
BIOMASS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CULTURES
DISEASES
ENERGY SOURCES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
NICOTIANA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PLANT CELLS
PLANT DISEASES
PLANTS
PROTEINS
RENEWABLE ENERGY SOURCES
SYNTHESIS
TOBACCO MOSAIC VIRUS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VIRUSES