Mutations in Escherichia coli altering an apurinic endonuclease, endonuclease II, and exonuclease III and their effect on in vivo sensitivity to methylmethanesulfonate

Journal Article · Tue Dec 13 04:00:00 EST 1977 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00644a037· OSTI ID:7277201

Kirtikar, D M; Cathcart, G R; White, J G; Ukstins, I; Goldthwait, D A

The levels of endonuclease II, an apurinic endonuclease, and exonuclease III in the parent strain (AB 1157) of Escherichia coli and in various mutants were determined by chromatography on DEAE-cellulose. AB 3027 and NH 5016 lacked endonuclease II and exonuclease III. BW 2001 lacked the apurinic endonuclease and exonuclease III while BW 2007, BW 9093, and BW 9059 lacked only exonuclease III. Deletion mutants BW 9101 and BW 9109 lacked all three enzymes. The latter mutants locate the genes for the two endonucleases in the region of exonuclease III (xth) of 38.2 min (White et al., 1976). All of the mutants which were sensitive to methylmethanesulfonate in vivo lacked exonuclease III, but not all mutants lacking exonuclease III were MMS sensitive. The deletion mutants and NH 5016 were the exceptions.

• Research Organization: Case Western Reserve Univ., Cleveland
• OSTI ID: 7277201
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 16:25; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
ENZYMES
ESCHERICHIA COLI
ESTERS
GENES
GENETIC MAPPING
METHYL METHANESULFONATE
MICROORGANISMS
MUTAGENS
MUTANTS
NUCLEASES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHOTRANSFERASES
SENSITIVITY
SULFONIC ACID ESTERS
TRANSFERASES