Interaction between radiation and drug damage in mammalian cells. II. The effect of actinomycin-D on the repair of sublethal radiation damage in plateau phase cells

Conference · Tue Jun 01 04:00:00 EDT 1976 · Cancer; (United States)

OSTI ID:7276012

Piro, A J; Taylor, C C; Belli, J A

The effect of actinomycin-D (AMD) on radiation damage repair was studied in plateau phase V79 Chinese hamster cells. Sublethal radiation damage repair, as demonstrated by survival fluctuations following two x-ray exposures separated by time was observed in our plateau phase cells. Plateau phase cells exposed to 0.01 to 0.04 ..mu..g/ml AMD (a nontoxic regimen to 8 hours) between x-ray exposures were less able to repair sublethal damage. If plateau phase cells were plated at low dilutions into fresh medium (conditions for resuming exponential growth) immediately after the first x-ray dose, and exposed to 0.01 to 0.04 ..mu..g/ml AMD until the second dose, inhibition of sublethal damage repair and additional cell killing were observed particularly at 0.04 ..mu..g/ml AMD. It is suggested that radiation-drug damage interactions should be studied in plateau phase cells and in cells resuming exponential growth after plateau phase (possibly analogous to ''recruitment''), as well as in exponential phase cultures.

• Research Organization: Harvard Medical School, Boston
• OSTI ID: 7276012
• Conference Information: Journal Name: Cancer; (United States) Journal Volume: 37:6
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTINOMYCIN
ANIMAL CELLS
ANTIBIOTICS
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
DRUGS
ELECTROMAGNETIC RADIATION
INHIBITION
IONIZING RADIATIONS
IRRADIATION
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
SUBLETHAL IRRADIATION
X RADIATION