X-ray potentiation of MuLV infection in vitro. [Murine leukemia virus]

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(76)90485-2· OSTI ID:7272862

Gelmann, E P; Niwa, O; Decleve, A; Kaplan, H S

Physical and chemical agents which cause DNA strand breakage enhance infection by DNA and RNA oncogenic viruses, presumably by facilitating the incorporation of viral genetic information into the host cell genome. X-irradiation has now been shown to potentiate infectious center formation by radiation leukemia virus (RadLV*). The potentiation was dose-dependent with a peak in the range of 300 to 450 rads. Doses in this range enhanced infectivity by a factor of about 1.5 to 2. X-irradiation also enhanced the infection of cells phenotypically nonpermissive for murine leukemia virus infection, but did not alter the characteristic 2-hit kinetics observed in such cells. Fractionation of the x-ray exposure into two doses separated by varying time intervals showed that the potentiation persisted up to 30 hr postinfection. The initial dose fraction, given at the time of infection, caused partial synchrony of the host cell population. A sharp peak of enhancement occurred when cells received the second dose fraction at a time when they had just completed one round of DNA synthesis and mitosis.

• Research Organization: Stanford Univ. School of Medicine, CA
• OSTI ID: 7272862
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 69; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
FRACTIONATED IRRADIATION
IONIZING RADIATIONS
IRRADIATION
MICROORGANISMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RNA
STRAND BREAKS
VIRUSES
X RADIATION