Bioactivation of the hepatocarcinogen N-hydroxy-2-acetylaminofluorene by sulfation in the rat liver changes during the cell cycle

Journal Article · Wed Jan 01 04:00:00 EST 1992 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(92)90014-G· OSTI ID:7262637

Gilissen, R A.H.J.; Meerman, J H.N. ^[1]

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1. Leiden Univ. (Netherlands)

Sulfation activity towards N-hydroxy-2-acetylaminofluorene and 4-nitrophenol was determined in male rat liver cytosol at several time points after partial hepatectomy corresponding to G{sub 1}-, S-, and M-phase. N-hydroxy-2-acetylaminofluorene sulfation activity decreased by 80% when hepatocytes entered the G{sub 1}-phase. This lower activity was maintained during the S-phase and M-phase, but was restored when hepatocytes entered the G{sub 0}-phase again. Sulfation activity towards 4-nitrophenol did not alter after hepatectomy. Various other cytosolic enzyme activities were determined after hepatectomy to investigate the specificity of the decrease in sulfation activity. Lactate dehydrogenase and glucose-6-phosphate dehydrogenase activities were increased in the S- and M-phase by maximally 80% and 60%, respectively. Glutathione-S-transferase and glutamate-pyruvate transminase activity did not alter during the cell cycle. These results indicate that sulfation of N-hydroxy-2-acetylaminofluorene in hepatocytes may depend on the phase of the cell cycle. The relevance of the finding is discussed in relation to the resistance of proliferating (pre)neoplastic hepatocytes to the toxic and mitoinhibitory effects of N-hydroxy-2-acetylaminofluorene.

• OSTI ID: 7262637
• Journal Information: Life Sciences; (United States), Journal Name: Life Sciences; (United States) Vol. 51:16; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
CARCINOGEN SCREENING
CELL CYCLE
CELL PROLIFERATION
CONDENSED AROMATICS
ENZYME ACTIVITY
ENZYMES
FLUORENE
HEPATECTOMY
HYDROCARBONS
HYDROXY COMPOUNDS
LIVER CELLS
MAMMALS
MEDICINE
METABOLIC ACTIVATION
NITRO COMPOUNDS
NITROPHENOL
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PHENOLS
PROTEINS
RATS
RODENTS
SCREENING
SENSITIVITY
SOMATIC CELLS
SURGERY
TRANSFERASES
VERTEBRATES