Role of deoxyribonucleic acid polymerase III in the repair of single-strand breaks produced in Escherichia coli deoxyribonucleic acid by gamma radiation

Journal Article · Wed Sep 01 00:00:00 EDT 1976 · J. Bacteriol.; (United States)

OSTI ID:7261941

Hamelin, C; Youngs, D A; Smith, K C

Cell survival, deoxyribonucleic acid (DNA) degradation, and the repair of DNA single-strand breaks were measured for Escherichia coli K-12 pol/sup +/, polAl, polC1026(ts), and polAl polC1026(ts) cells after /sup 137/Cs ..gamma.. irradiation. The results indicate that DNA polymerase III is required for growth medium-dependent (type III) repair in polA/sup +/ or polA cells and is necessary for growth medium-independent (type II) repair in polA cells. In pol/sup +/ or polC cells, DNA polymerase I performs type II repair efficiently. The relative deficiencies of each of these strains in DNA repair generally correlate with their relative sensitivities to cell killing and with the extent of DNA degradation observed.

• Research Organization: Stanford Univ., CA
• OSTI ID: 7261941
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 127:3; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL KILLING
DNA
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
GAMMA RADIATION
IONIZING RADIATIONS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POLYMERASES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
STRAND BREAKS