DNA repair in DNA-polymerase-deficient mutants of Escherichia coli
Conference
·
OSTI ID:4125893
- ed.
Escherichia coli mutants deficient in DNA polymerase I, in DNA polymerases I and II, or in DNA polymerase III can efficiently and completely execute excision-repair and postreplication repair of the uv-damaged DNA at 30$sup 0$C and 43$sup 0$C when assayed by alkaline sucrose gradients. Repair by Pol I$sup -$ and Pol I$sup -$, Pol II$sup -$ cells is inhibited by 1-$beta$-D- arabinofuranosylcytosine (araC) at 43$sup 0$C but not at 30$sup 0$C, whereas that by Pol III$sup -$ cells is insensitive to araC at any temperature. Thus, either Pol I or Pol III is required for complete and efficient repair, and in their absence Pol II mediates a limited, incomplete dark repair of uv-damaged DNA. (auth)
- Research Organization:
- Univ. of California, San Diego
- NSA Number:
- NSA-33-012292
- OSTI ID:
- 4125893
- Country of Publication:
- United States
- Language:
- English
Similar Records
Repair of damaged DNA in vivo. Annual progress report, May 9, 1975--May 10, 1976. [Ultraviolet radiation, Escherichia coli]
UV-induced ubiquitination of RNA polymerase II: A novel modification deficient in Cockayne syndrome cells
Role of DNA polymerase I in excision-repair
Technical Report
·
Wed Dec 31 23:00:00 EST 1975
·
OSTI ID:7212851
UV-induced ubiquitination of RNA polymerase II: A novel modification deficient in Cockayne syndrome cells
Journal Article
·
Tue Oct 15 00:00:00 EDT 1996
· Proceedings of the National Academy of Sciences of the United States of America
·
OSTI ID:471597
Role of DNA polymerase I in excision-repair
Conference
·
Tue Dec 31 23:00:00 EST 1974
·
OSTI ID:4126147
Related Subjects
*DNA-- BIOLOGICAL REPAIR
*ESCHERICHIA COLI-- BIOLOGICAL RADIATION EFFECTS
*POLYMERASES
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
MUTANTS
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
ULTRAVIOLET RADIATION
*ESCHERICHIA COLI-- BIOLOGICAL RADIATION EFFECTS
*POLYMERASES
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
MUTANTS
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
ULTRAVIOLET RADIATION