DNA repair in DNA-polymerase-deficient mutants of Escherichia coli

Conference · Tue Dec 31 23:00:00 EST 1974

OSTI ID:4125893

Smith, D W; Tait, R C; Harris, A L; Hanawalt, P C ^[1]

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1. ed.

Escherichia coli mutants deficient in DNA polymerase I, in DNA polymerases I and II, or in DNA polymerase III can efficiently and completely execute excision-repair and postreplication repair of the uv-damaged DNA at 30$sup 0$C and 43$sup 0$C when assayed by alkaline sucrose gradients. Repair by Pol I$sup -$ and Pol I$sup -$, Pol II$sup -$ cells is inhibited by 1-$beta$-D- arabinofuranosylcytosine (araC) at 43$sup 0$C but not at 30$sup 0$C, whereas that by Pol III$sup -$ cells is insensitive to araC at any temperature. Thus, either Pol I or Pol III is required for complete and efficient repair, and in their absence Pol II mediates a limited, incomplete dark repair of uv-damaged DNA. (auth)

• Research Organization: Univ. of California, San Diego
• NSA Number: NSA-33-012292
• OSTI ID: 4125893
• Country of Publication: United States
• Language: English

Related Subjects

*DNA-- BIOLOGICAL REPAIR
*ESCHERICHIA COLI-- BIOLOGICAL RADIATION EFFECTS
*POLYMERASES
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
MUTANTS
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
ULTRAVIOLET RADIATION