Role of DNA polymerase I in excision-repair

Conference · Tue Dec 31 23:00:00 EST 1974

OSTI ID:4126147

Glickman, B W; Hanawalt, P C ^[1]

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1. ed.

The ability of three different DNA polymerase I mutants of Escherichia coli to carry out excision-repair was examined. Strains having the same genetic origin but carrying either the polA1, polA107, resA1, or pol$sup +$ alleles were compared. The rate of ultraviolet-induced dimer excision was slightly reduced, relative to that found in Pol$sup +$ strains, in the PolA1 strain; greatly reduced in the PolA107 strain; and found not to occur in the ResA1 strain. Ultraviolet-light-induced repair synthesis as determined by the ultraviolet- stimulated incorporation of $sup 3$H-labeled 5-bromo-2'-deoxyuridine into DNA of the parental density showed that the polA1 mutation resulted in an increase in repair replication, while the presence of the polA107 allele caused a reduction in the amount of repair synthesis relative to that of the Pol$sup +$ strain. The ResA1 strain, however, showed no ultraviolet stimulation of the incorporation of the density label. These observations indicate that DNA polymerase I plays a key role in the excision-repair process in E. coli. (auth)

• Research Organization: Leiden State Univ.
• NSA Number: NSA-33-012270
• OSTI ID: 4126147
• Country of Publication: United States
• Language: English

Related Subjects

*DNA-- BIOLOGICAL REPAIR
*ESCHERICHIA COLI-- BIOLOGICAL RADIATION EFFECTS
*POLYMERASES-- BIOLOGICAL EFFECTS
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
BIOSYNTHESIS
BUDR
DIMERS
MUTANTS
N48110 --Life Sciences--Radiation Effects on Biochemicals--In-Vitro
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
ULTRAVIOLET RADIATION