Pervaporative butanol fermentation by Clostridium acetobutylicum B18

Journal Article · Fri Apr 15 04:00:00 EDT 1994 · Biotechnology and Bioengineering; (United States)

DOI:https://doi.org/10.1002/bit.260431011· OSTI ID:7261916

Geng, Q; Park, C H ^[1]

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1. Univ. of Minnesota, St. Paul, MN (United States). Dept. of Agricultural Engineering

Extractive acetone-butanol-ethanol (ABE) fermentation was carried out successfully using pervaporation and a low-acid-producing Clostridium acetobutylicum B18. A pervaporation module with 0.17 m[sup 2] of surface area was made of silicone membrane of 240 [mu]m thickness. Pervaporation experiments using make-up solutions showed that butanol and acetone fluxes increased linearly with their concentrations in the aqueous phase. Fickian diffusion coefficients were constants for fixed air flow rates, and increased at higher sweep air flow rates. During batch and fed-batch fermentation, pervaporation at an air flow rate of 8 L/min removed butanol and acetone efficiently. Butanol concentration was maintained below 4.5 g/L even though Clostridium acetobutylicum B18 produced butanol steadily. Pervaporation could not remove organic acids efficiently, but organic acids did not accumulate because strain B18 produced little organic acid and recycled added organic acids efficiently. With pervaporation, glucose consumption rate increased compared to without pervaporation, and up to 160 g/L of glucose was consumed during 80 h. Cell growth was not inhibited by possible salt accumulation or oxygen diffusion through the silicone tubing. The culture volume was maintained relatively constant during fed-batch operation because of an offsetting effect of water and product removal by pervaporation and addition of nutrient supplements.

• OSTI ID: 7261916
• Journal Information: Biotechnology and Bioengineering; (United States), Journal Name: Biotechnology and Bioengineering; (United States) Vol. 43:9; ISSN BIBIAU; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090900* -- Biomass Fuels-- Processing-- (1990-)
ALCOHOLS
ALDEHYDES
BACTERIA
BIOCONVERSION
BIOSYNTHESIS
BUTANOLS
CARBOHYDRATES
CLOSTRIDIUM
CLOSTRIDIUM ACETOBUTYLICUM
FERMENTATION
GLUCOSE
GROWTH
HEXOSES
HYDROXY COMPOUNDS
METHANOGENIC BACTERIA
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
SYNTHESIS