In situ extractive fermentation of acetone and butanol
- Univ. of California, Berkeley (USA)
The productivity of the acetone-butanol fermentation was increased by continuously removing acetone and butanol from the fermentation broth during fed-batch culture. Whole broth containing viable cells of Clostridium acetobutylicum was cycled to a Karr reciprocating plate extraction column in which acetone and butanol were extracted into oleyl alcohol flowing counter-currently through the column. By continuously removing these toxic metabolites from the broth, end product inhibition was reduced, and a concentrated feed solution containing 300 g/L glucose was fermented at an overall butanol productivity of 1.0 g/L h, 70% higher than the productivity of normal batch fermentation. The continuous extraction process provides flexible operation and lends itself to process scale-up.
- OSTI ID:
- 5332833
- Journal Information:
- Biotechnology and Bioengineering; (USA), Vol. 31:2; ISSN 0006-3592
- Country of Publication:
- United States
- Language:
- English
Similar Records
Continuous direct solvent extraction of butanol in a fermenting fluidized-bed bioreactor with immobilized Clostridium acetobutylicum
Continuous direct solvent extraction of butanol in a fermenting fluidized-bed bioreactor with immobilized Clostridium acetobutylicum
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETONE
BIOSYNTHESIS
TOXICITY
BUTANOLS
GLUCOSE
FERMENTATION
CLOSTRIDIUM ACETOBUTYLICUM
ECONOMICS
EXTRACTION
INHIBITION
METABOLITES
REMOVAL
ALCOHOLS
ALDEHYDES
BACTERIA
BIOCONVERSION
CARBOHYDRATES
CLOSTRIDIUM
HEXOSES
HYDROXY COMPOUNDS
KETONES
METHANOGENIC BACTERIA
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
SEPARATION PROCESSES
SYNTHESIS
090900* - Biomass Fuels- Processing- (1990-)
560300 - Chemicals Metabolism & Toxicology