In situ extractive fermentation of acetone and butanol

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Biotechnology and Bioengineering; (USA)

DOI:https://doi.org/10.1002/bit.260310207· OSTI ID:5332833

Roffler, S R; Blanch, H W; Wilke, C R ^[1]

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1. Univ. of California, Berkeley (USA)

The productivity of the acetone-butanol fermentation was increased by continuously removing acetone and butanol from the fermentation broth during fed-batch culture. Whole broth containing viable cells of Clostridium acetobutylicum was cycled to a Karr reciprocating plate extraction column in which acetone and butanol were extracted into oleyl alcohol flowing counter-currently through the column. By continuously removing these toxic metabolites from the broth, end product inhibition was reduced, and a concentrated feed solution containing 300 g/L glucose was fermented at an overall butanol productivity of 1.0 g/L h, 70% higher than the productivity of normal batch fermentation. The continuous extraction process provides flexible operation and lends itself to process scale-up.

• OSTI ID: 5332833
• Journal Information: Biotechnology and Bioengineering; (USA), Journal Name: Biotechnology and Bioengineering; (USA) Vol. 31:2; ISSN 0006-3592; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090900* -- Biomass Fuels-- Processing-- (1990-)
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETONE
ALCOHOLS
ALDEHYDES
BACTERIA
BIOCONVERSION
BIOSYNTHESIS
BUTANOLS
CARBOHYDRATES
CLOSTRIDIUM
CLOSTRIDIUM ACETOBUTYLICUM
ECONOMICS
EXTRACTION
FERMENTATION
GLUCOSE
HEXOSES
HYDROXY COMPOUNDS
INHIBITION
KETONES
METABOLITES
METHANOGENIC BACTERIA
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
REMOVAL
SACCHARIDES
SEPARATION PROCESSES
SYNTHESIS
TOXICITY