Complement facilitates macrophage phagocytosis of inhaled iron particles but has little effect in mediating silica-induced lung inflammatory and clearance responses

Journal Article · Sun Dec 01 04:00:00 EST 1991 · Environmental Research; (United States)

DOI:https://doi.org/10.1016/S0013-9351(05)80008-8· OSTI ID:7260246

Warheit, D B; Carakostas, M C; Bamberger, J R; Hartsky, M A ^[1]

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1. E.I. de Pont de Nemours and Co., Newark, DE (United States)

The present studies were undertaken to investigate the role of complement in mediating pulmonary inflammation and/or phagocytosis as a function of particle clearance in rats exposed to silica or carbonyl iron (CI) particles. Both particle types were shown to be weak activators of serum complement in vitro. In these studies, normal and complement-depressed (CVFD-treated) rats were exposed to aerosols of Ci or silica particles for 6 hr at 100 mg/m{sup 3}. Following exposure, alveolar fluids and cells from sham and dust-exposed animals were recovered by bronchoalveolar lavage (BAL) at several time periods postexposure and measured for a variety of biochemical and cellular indices. In addition, pulmonary macrophages were cultured and studied for morphology and phagocytosis. The authors results showed that CI exposure did not produce cellular or biochemical indices of pulmonary inflammation, either in normal or complement-depleted rats. However, fewer phagocytic macrophages were recovered from the lungs of CVF-treated, CI-exposed rats than from normal exposed animals. In contrast, silica inhalation produced a sustained PMN inflammatory response in the lungs of exposed rats, measured up through 1 month postexposure, along with significant increases in BAL fluid levels of LDH, protein, and alkaline phosphatase and deficits in pulmonary macrophage phagocytic functions.

• OSTI ID: 7260246
• Journal Information: Environmental Research; (United States), Journal Name: Environmental Research; (United States) Vol. 56:2; ISSN ENVRA; ISSN 0013-9351
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE PHOSPHATASE
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CHALCOGENIDES
CLEARANCE
COMPLEMENT
CONNECTIVE TISSUE CELLS
ELEMENTS
ENZYMES
ESTERASES
EXCRETION
HYDROLASES
INFLAMMATION
INHALATION
INTAKE
IRON
KINETICS
LUNG CLEARANCE
LUNGS
MACROPHAGES
MAMMALS
METALS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PARTICLES
PARTICULATES
PATHOLOGICAL CHANGES
PHAGOCYTES
PHAGOCYTOSIS
PHOSPHATASES
PROTEINS
RATS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SILICON COMPOUNDS
SILICON OXIDES
SOMATIC CELLS
SYMPTOMS
TOXICITY
TRANSITION ELEMENTS
VERTEBRATES