Blockade of immune tolerance induction by puromycin aminonucleoside. [Mice]

Journal Article · Wed Jun 15 04:00:00 EDT 1977 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(77)90027-2· OSTI ID:7254647

Maserang, D L; Hyde, R M

A transfer system was used to explore the metabolic requirements for tolerance induction to human gamma globulin in the murine thymocyte. CBF1 (BALB/c x C57BL/6) mice were treated with a metabolic inhibitor and deaggregated HGG. Twenty-four hours later these mice were used as donors of thymus cells for irradiated recipients reconstituted with normal bone marrow cells. After a course of immunogenic HGG, the recipient animals were sacrificed and their immunocompetence assessed by use of the hemolytic plaque assay. It was determined that puromycin aminonucleoside (PAN), an inhibitor of RNA and protein synthesis, was capable of obviating tolerance induction in this system. This obviation was found to be temporal, and was not due to increased activity of the reticuloendothelial system. Radioautography revealed that these observed effects with PAN were not due to interference with specific interactions at the cellular membrane. The possible role of PAN in this system is discussed.

• Research Organization: Univ. of Oklahoma, Oklahoma City
• OSTI ID: 7254647
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 31:2; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
DRUGS
GLOBULINS
GLOBULINS-GAMMA
IMMUNE REACTIONS
MAMMALS
METABOLISM
MICE
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
PROTEINS
PUROMYCIN
RIBOSIDES
RODENTS
SOMATIC CELLS
THYMOCYTES
VERTEBRATES