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Recombination in Saccharomyces cerevisiae: a DNA repair mutation associated with elevated mitotic gene conversion. [X and UV radiation]

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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A mutant haploid strain of Saccharomyces cerevisiae has been isolated that is sensitive to the alkylating agent methyl methanesulfonate at a concentration of 0.01% (vol/vol). The strain also shows sensitivities to x-rays and ultraviolet light, which cosegregate with sensitivity to methyl methanesulfonate as a single gene defect. An analysis of the sensitivity to ultraviolet light indicates that the mutation interferes with the excision of pyrimidine dimers. Diploids homozygous for the mutant gene exhibit elevated frequencies of spontaneous mitotic recombination at the ade6 locus. The results indicate that all the events are due to gene conversion. Mitotic recombination was also found to be elevated for three loci other than ade6. Thus, the recombinational effect seems not to be locus specific. Linkage and allelism tests indicate that the mutation is an allele of the known radiation-sensitive gene rad18. The various effects of this new rad18 allele (rad18-3) are discussed in terms of a defect in DNA repair mechanisms.
Research Organization:
Univ. of Washington, Seattle
OSTI ID:
7254625
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 73:8; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOMASS
DNA
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
ESTERS
FUNGI
GENE RECOMBINATION
GENES
IONIZING RADIATIONS
METHYL METHANESULFONATE
MICROORGANISMS
MUTAGENS
MUTANTS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
RENEWABLE ENERGY SOURCES
REPAIR
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SULFONIC ACID ESTERS
ULTRAVIOLET RADIATION
X RADIATION
YEASTS