Structural studies of. cap alpha. -bungarotoxin. 1. Sequence-specific /sub 1/H NMR resonance assignments
The authors report the complete sequence-specific assignment of the backbone resonances and most of the side-chain resonances in the /sup 1/H NMR spectrum of ..cap alpha..-bungarotoxin by two-dimensional NMR. Problems with resonance overlap were resolved with the assistance of the HRNOESY experiment described in an accompanying paper. Significant differences exist between the solution structure described here and the crystal structure of ..cap alpha..-bungarotoxin, on the basis of the proton to proton distances obtained by nuclear Overhauser enhancement spectroscopy (NOESY) and the corresponding distances from the X-ray crystal structure. These differences include a larger ..beta..-sheet in solution and a different orientation of the invariant tryptophan, Trp-28, making the solution structure more consistent with the crystal structure of the homologous neurotoxin ..cap alpha..-cobratoxin. Four errors in the order of the amino acids in the primary sequence were indicated by the NMR data. These errors were confirmed by chemical means, as described in an accompanying paper.
- Research Organization:
- Univ. of California, San Francisco (USA)
- OSTI ID:
- 7253194
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 27:8; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACID SEQUENCE
AMINO ACIDS
ANTIGENS
AROMATICS
AZAARENES
AZOLES
CARBOXYLIC ACIDS
CHEMICAL SHIFT
COHERENT SCATTERING
DIFFRACTION
HEAVY WATER
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INDOLES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MATERIALS
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OVERHAUSER EFFECT
OXYGEN COMPOUNDS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
PYRROLES
RESONANCE
SCATTERING
STABLE ISOTOPES
TOXIC MATERIALS
TOXINS
TRYPTOPHAN
WATER
X-RAY DIFFRACTION