Structural studies of. cap alpha. -bungarotoxin. 2. /sub 1/H NMR assignments via an improved relayed coherence transfer nuclear Overhauser enhancement experiment

Journal Article · Tue Apr 19 00:00:00 EDT 1988 · Biochemistry; (United States)

OSTI ID:7142881

Basus, V J; Scheek, R M

Complete sequence-specific assignments of the /sup 1/H NMR spectrum of bungarotoxin were reported in a previous paper. The assignment was significantly aided by the use of the homonuclear Hartman-Hahn relayed coherence transfer nuclear Overhauser enhancement spectroscopy experiment (HRNOESY) which we present here, as a modification of relayed coherence transfer nuclear Overhauser enhancement spectroscopy (relayed NOESY). As shown here, HRNOESY resolves problems of proton resonance overlap especially in extended chain conformations as found in ..beta..-sheets.

• Research Organization: Univ. of California, San Francisco (USA)
• OSTI ID: 7142881
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 27:8; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACID SEQUENCE
ANTIGENS
BARYONS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HEAVY WATER
HYDROGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MATERIALS
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
OVERHAUSER EFFECT
OXYGEN COMPOUNDS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
PROTONS
RESONANCE
STABLE ISOTOPES
TOXIC MATERIALS
TOXINS
WATER