Chronic ethanol consumption inhibits repair of dimethylnitrosamine-induced DNA alkylation

Journal Article · Fri Apr 15 04:00:00 EDT 1988 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/S0006-291X(88)80731-9· OSTI ID:7252539

Mufti, S I; Salvagnini, M; Lieber, C S; Garro, A J

Chronic ethanol consumption causes a DNA repair deficiency. This was demonstrated in Sprague-Dawley rats injected with /sup 14/C-labeled dimethylnitrosamine after being pair-fed isocaloric, ethanol, or carbohydrate control diets for 4 weeks. Hepatic DNA was isolated from rats killed at intervals over a 36 hour period after administration of the nitrosamine and concentrations of alkylated guanine derivatives were measured. While N7-methylguanine was lost at equivalent rates from the DNA of both diet groups, 06methylguanine, a promutagenic lesion, persisted at higher levels for longer periods of time in the DNA from the alcohol-fed animals.

• Research Organization: Mount Sinai School of Medicine, New York, NY (USA)
• OSTI ID: 7252539
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 152:1; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
AMINES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
CHRONIC EXPOSURE
DIGESTIVE SYSTEM
DNA
DNA REPAIR
ETHANOL
GLANDS
HYDROXY COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
METHYLATION
NITROSAMINES
NITROSO COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RATS
RECOVERY
REPAIR
RODENTS
TRACER TECHNIQUES
VERTEBRATES