Investigation of the mechanism of iron acquisition by the marine bacterium Alteromonas luteoviolaceus: Characterization of siderophore production
- Univ. of California, Santa Barbara (United States)
Iron availability in the ocean ranges from one to four orders of magnitude below typical growth requirements of bacteria. The discrepancy between Fe availability and requirements raises questions about the mechanisms that marine bacteria use to sequester Fe{sup 3+}. Surprisingly little is known about the siderophores produced by marine bacteria. Growth conditions of an open-ocean bacterial isolate, Alteromonas luteoviolaceus, were investigated to determine the conditions which enhance siderophore production. Methods to isolate and purify the siderophores were determined. The siderophores produced by A. luteoviolaceus were partially characterized by mass spectral analysis, amino acid analysis, qualitative analytical tests, chemical degradation, and nuclear magnetic resonance. A new set of outer membrane proteins was also produced when the bacterium was grown under Fe-limited conditions.
- OSTI ID:
- 7250541
- Journal Information:
- Limnology and Oceanography; (United States), Vol. 36:8; ISSN 0024-3590
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
29 ENERGY PLANNING
POLICY AND ECONOMY
BACTERIA
GROWTH
IRON
BIOLOGICAL AVAILABILITY
SEAS
BIOGEOCHEMISTRY
AMINO ACID SEQUENCE
BIOSYNTHESIS
CARBON SINKS
MASS SPECTRA
MEMBRANE PROTEINS
NMR SPECTRA
NUTRIENTS
QUALITATIVE CHEMICAL ANALYSIS
CHEMICAL ANALYSIS
CHEMISTRY
ELEMENTS
GEOCHEMISTRY
METALS
MICROORGANISMS
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
PROTEINS
SINKS
SPECTRA
SURFACE WATERS
SYNTHESIS
TRANSITION ELEMENTS
540320* - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)
560300 - Chemicals Metabolism & Toxicology
290301 - Energy Planning & Policy- Environment
Health
& Safety- Regional & Global Environmental Aspects- (1992-)