Genetics of bacteria that utilize one-carbon compounds. Progress report, November 1, 1985-October 31, 1986
Three major regions of the M. organophilum strain XX genome are required for expression of active methanol dehydrogenase (MDH). Clustered within these regions are at least eleven genes. Western blots indicate that variable amounts of inactive MDH are produced by different mutants. With the exception of group V-C, the level of inactive MDH is consistent for mutants within any one of the eleven subgroups. Group V-C contains the structural gene. Identification of this gene has been verified by an identification of a fusion protein that cross reacts with anti-MDH antibodies. Work has commenced to define the location and sequence of the structural gene regulatory sequences, which reside on a 2.5 kb SmaI restriction fragment. An additional four unlinked regions of the M. organophilum that encode enzymes required for the assimilation of formaldehyde have also been identified. 8 refs., 5 figs., 5 tabs.
- Research Organization:
- Minnesota Univ., Navarre (USA). Gray Freshwater Biological Inst.
- DOE Contract Number:
- AC02-82ER12029
- OSTI ID:
- 7246986
- Report Number(s):
- DOE/ER/12029-T1; ON: DE87001924
- Country of Publication:
- United States
- Language:
- English
Similar Records
Genetic and physical analyses of Methylobacterium organophilum XX genes encoding methanol oxidation
Nucleotide sequence and transcriptional start site of the Methylobacterium organophilum XX methanol dehydrogenase structural gene