Genetics of bacteria that utilize one carbon compounds: Final report, March 1, 1982-February 29, 1988
Broad host range plasmid vectors useful for cloning genes from bacteria that grow on methane and methanol were constructed. We have cloned and mapped nineteen genes required for the growth of Methylobacterium organophilum strain XX on methanol. Nineteen genes were found in seven linkage groups on the M. organophilum genome and were separated by 40 kb or more. Eleven genes were required for the synthesis of methanol dehydrogenase (MDH) and were located in three unlinked gene clusters. The MDH structural gene was localized on a 2.5 kb DNA fragment. The gene was sequenced and contains a 175 bp untranslated leader sequence, a signal sequence and the structural gene. MDH messenger RNA (mRNA) has a half life of approximately 20 min. and is present at approximately 2% of the cellular mRNA. The structural gene for the ..gamma.. subunit of methane monoxygenases has been cloned from Methylosporovibrio. Methane monooxygenase subunits have been purified by Prof. J. Lipscomb's laboratory and are being sequenced to construct DNA probes to identify cloned subunit genes. New facultative methylotrophic bacteria were isolated and characterized. Several amino acid auxotrophs have been isolated. 11 refs.
- Research Organization:
- Minnesota Univ., Navarre (USA). Gray Freshwater Biological Inst.
- DOE Contract Number:
- AC02-82ER12029
- OSTI ID:
- 5496021
- Report Number(s):
- DOE/ER/12029-T2; ON: DE88006376
- Country of Publication:
- United States
- Language:
- English
Similar Records
Nucleotide sequence and transcriptional start site of the Methylobacterium organophilum XX methanol dehydrogenase structural gene
Genetics of bacteria that utilize one-carbon compounds. Progress report, November 1, 1985-October 31, 1986
Related Subjects
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550200 -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALKANES
BACTERIA
CATABOLISM
CLONING
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
ENZYMES
GENETIC MAPPING
HYDROCARBONS
HYDROXY COMPOUNDS
MAPPING
METABOLISM
METHANE
METHANOL
METHANOTROPHIC BACTERIA
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PROGRESS REPORT
RNA PROCESSING
STRUCTURAL CHEMICAL ANALYSIS