A comparative study of low pH stress in E. coli and S. typhimurium, and a comparative study of the inducibility of lysyl-tRNA synthetase in the enterobacteriaceae
Thesis/Dissertation
·
OSTI ID:7246052
Lysyl-tRNA synthetase (LRS) in Escherichia coli is coded by two genes, one constitutive, and the other inducible. The commonness of inducibility of this enzyme in prokaryotes was first tested in eight members of the Enterobacteriaceae using culture conditions known to induce it in E. coli. LRS was found to be inducible in Salmonella Typhimurium, Citrobacter freundii, Klebsiella pneumoniae and Enterobacter aerogenes, but not in Serratia marcescens, Proteus mirabilis, Proteus vulgaris or Morganella morganii. The results also indicated that LRS was not induced in E. coli grown in defined medium (SMM) at an external pH (pH{sub 0}) of 5.0, whereas, it was induced in S. typhimurium under this condition. Further investigation of low pH{sub 0} induced behavior in E. coli and S. typhimurium by quantitation of H{sub 2} {sup 35}SO{sub 4} labeled proteins from two dimensional polyacrylamide gels of whole cell sonic extracts showed that at least twenty proteins were induced from 2- to 16-fold in S. typhimurium grown at pH{sub 0} 5.0 or shifted from growth at pH{sub 0} 7.0 to 5.0. Internal pH (pH{sub i}) changes occurring during steady state growth at low pH{sub 0}, and on shifting from pH{sub 0} 7.0 to 5.0, were measured using {sup 14}C-benzoic acid uptake.
- Research Organization:
- Saint John's Univ., Jamaica, NY (USA)
- OSTI ID:
- 7246052
- Country of Publication:
- United States
- Language:
- English
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Conference
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Fri May 01 00:00:00 EDT 1987
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BENZOIC ACID
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL STRESS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
ESCHERICHIA COLI
EVEN-ODD NUCLEI
GENES
GROWTH
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGASES
LIGHT NUCLEI
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PH VALUE
RADIOISOTOPES
SALMONELLA
SALMONELLA TYPHIMURIUM
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BENZOIC ACID
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL STRESS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
ESCHERICHIA COLI
EVEN-ODD NUCLEI
GENES
GROWTH
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGASES
LIGHT NUCLEI
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PH VALUE
RADIOISOTOPES
SALMONELLA
SALMONELLA TYPHIMURIUM
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES