Phagocytic and chemiluminescent responses of mouse peritoneal macrophages to living and killed Salmonella typhimurium and other bacteria
Journal Article
·
· Infect. Immun.; (United States)
OSTI ID:5502911
In the presence of luminol, resident as well as thioglycolate-induced and immunized macrophages emitted chemiluminescence more efficiently when the cells were exposed to living Salmonella typhimurium than when they were exposed to the same bacterium killed by ultraviolet light or heat. This phenomenon was observed whether or not the bacterium was opsonized. The different response to living and killed bacteria was also found with Escherichia coli, Pseudomonas aeruginosa, Proteus morganii, and Enterobacter aerogenes, but not with Shigella sonnei, Klebsiella pneumoniae, and Propionibacterium acnes. The results suggest that macrophages respond better to living, motile bacteria than to nonmotile or killed bacteria. The experimental results obtained with motility mutants of S. typhimurium, E. coli, and P. aeruginosa confirm that macrophages exposed to the motile bacteria emit chemiluminescence more efficiently and ingest the motile bacteria at a much faster rate than the nonmotile bacteria.
- OSTI ID:
- 5502911
- Journal Information:
- Infect. Immun.; (United States), Journal Name: Infect. Immun.; (United States) Vol. 32:3; ISSN INFIB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550300 -- Cytology
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BACTERIA
CELL KILLING
CHEMILUMINESCENCE
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ENERGY
ESCHERICHIA COLI
HEAT
LUMINESCENCE
MACROPHAGES
MAMMALS
MICE
MICROORGANISMS
PHAGOCYTES
PHAGOCYTOSIS
PROTEUS
PSEUDOMONAS
RADIATIONS
RADIOINDUCTION
RODENTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SOMATIC CELLS
ULTRAVIOLET RADIATION
VERTEBRATES
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BACTERIA
CELL KILLING
CHEMILUMINESCENCE
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ENERGY
ESCHERICHIA COLI
HEAT
LUMINESCENCE
MACROPHAGES
MAMMALS
MICE
MICROORGANISMS
PHAGOCYTES
PHAGOCYTOSIS
PROTEUS
PSEUDOMONAS
RADIATIONS
RADIOINDUCTION
RODENTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SOMATIC CELLS
ULTRAVIOLET RADIATION
VERTEBRATES