Trypsin-induced ATPase activity in potato mitochondria
Potato mitochondria (Solanum tuberosum var. Russet Burbank), which readily phosphorylate ADP in oxidative phosphorylation, show low levels of ATPase activity which is stimulated neither by Mg/sup 2 +/, 2,4-dinitrophenol, incubation with respiratory substrates, nor disruption by sonication or treatment with Triton X-100, individually or in concert. Treatment of disrupted potato mitochondria with trypsin stimulates Mg/sup 2 +/-dependent, oligomycin-sensitive ATPase activity 10- to 15-fold, suggesting the presence of an ATPase inhibitor protein. Trypsin-induced ATPase activity was unaffected by uncoupler. Oligomycin-sensitive ATPase activity decreases as exposure to trypsin is increased. Incubation at alkaline pH or heating at 60/sup 0/C for 2 minutes also activates ATPase of sonicated potato mitochondria. Disruption of cauliflower (Brassica oleracea), red sweet potato (Ipomoea batatas), and carrot (Daucus carota) mitochondria increases ATPase activity, which is further enhanced by treatment with trypsin. The significance of the tight association of the inhibitor protein and ATPase in potato mitochondria is not clear.
- Research Organization:
- Univ. of California, Los Angeles, CA (United States)
- DOE Contract Number:
- AT(04-3)-34
- OSTI ID:
- 7245659
- Journal Information:
- Plant Physiol.; (United States), Vol. 57:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ATP-ASE
BIOCHEMICAL REACTION KINETICS
MITOCHONDRIA
TRYPSIN
ADP
ALKALOIDS
ANTIBIOTICS
ATP
BRASSICA
CARROTS
DINITROPHENOL
INHIBITION
MAGNESIUM
PHOSPHORYLATION
POTATOES
PROTEINS
SOUND WAVES
SUBSTRATES
VEGETABLES
ALKALINE EARTH METALS
AROMATICS
BIOMASS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DRUGS
ELEMENTS
ENERGY SOURCES
ENZYMES
ESTERASES
FOOD
HYDROLASES
HYDROXY COMPOUNDS
KINETICS
METALS
NITRO COMPOUNDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PEPTIDE HYDROLASES
PHENOLS
PHOSPHATASES
PLANTS
REACTION KINETICS
RENEWABLE ENERGY SOURCES
TUBERS
550300* - Cytology
550200 - Biochemistry