Site of origin and mechanism of action of adenosine in the frog sympathetic ganglion
Thesis/Dissertation
·
OSTI ID:7244622
The contribution of pre and postsynaptic activation on the release of {sup 3}H-purines was studied in the isolated sympathetic paravertebral ganglion of the frog. Preganglionic stimulation induced an overall release of {sup 3}H-purines. This release is blocked by atropine and curare and can be induced by carbachol and antidromic stimulation. Analyses of the effluent by anion exchange chromatography and by HPLC showed that the non-nucleotide fractions constituted most of the counts released. Hence, nucleosides are the main products released by the ganglion and did not arise from hydrolysis of extracellular ATP. We studied the effect of synaptic activity on tritiated inositol release (IR). This release did not change during orthodromic stimulation. However, upon cessation of the stimulation, release increased rapidly and remained elevated for at least 45 minutes. This increase in IR was reduced by suffusion of the ganglia with either acetylcholine or adenosine.
- Research Organization:
- Virginia Univ., Charlottesville, VA (USA)
- OSTI ID:
- 7244622
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
AROMATICS
AZAARENES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CARBOHYDRATES
CHEMISTRY
CHROMATOGRAPHY
FROGS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INOSITOL
INOSITOLS
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
KINETICS
LIQUID COLUMN CHROMATOGRAPHY
MONOSACCHARIDES
NERVE CELLS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
REACTION KINETICS
RIBOSIDES
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
AROMATICS
AZAARENES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CARBOHYDRATES
CHEMISTRY
CHROMATOGRAPHY
FROGS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INOSITOL
INOSITOLS
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
KINETICS
LIQUID COLUMN CHROMATOGRAPHY
MONOSACCHARIDES
NERVE CELLS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
REACTION KINETICS
RIBOSIDES
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES