Adenosine (ADO) released during orthodromic stimulation of the frog sympathetic ganglion inhibits phosphatidylinositol turnover (PI) associated with synaptic transmission

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6936899

Curnish, R; Bencherif, M; Rubio, R; Berne, R M

The authors have previously demonstrated that /sup 3/H-purine release was enhanced during synaptic activation of the prelabelled frog sympathetic ganglion. In addition, during orthodromic stimulation, there is an increased /sup 3/H-inositol release (an index of PI) that occurs during the poststimulation period and not during the period of stimulation. They hypothesized that endogenous ADO inhibits PI turnover during orthodromic stimulation. To test this hypothesis (1) they performed experiments to directly measure ADO release in the extracellular fluid by placing the ganglion in a 5 ..mu..l drop of Ringer's and let it come to equilibrium with the interstitial fluid, (2) they destroyed endogenous ADO by suffusing adenosine deaminase (ADA) during the stimulation period. Their results show (1) orthodromic stimulation increases release of ADO into the bathing medium, (2) ADA induced an increase of PI during the stimulation period in contrast to an increase seen only during the poststimulation period when ADA was omitted. They conclude that there is dual control of PI during synaptic activity, a stimulatory effect (cause unknown) and a short lived inhibitory effect that is probably caused by adenosine.

• Research Organization: Univ. of Virginia School of Medicine, Charlottesville
• OSTI ID: 6936899
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
AMIDINASES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CHEMISTRY
ENZYMES
ESTERS
FROGS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
METABOLISM
NERVES
NERVOUS SYSTEM
NON-PEPTIDE C-N HYDROLASES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
RIBOSIDES
SECRETION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES