Apparatus for eliminating background interference in fluorescence measurements
Patent
·
OSTI ID:7242979
The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle. 8 figs.
- DOE Contract Number:
- W-7405-ENG-36
- Assignee:
- Dept. of Energy, Washington, DC (United States)
- Patent Number(s):
- US 4573796; A
- Application Number:
- PPN: US 6-568768
- OSTI ID:
- 7242979
- Country of Publication:
- United States
- Language:
- English
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