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Chromosome characterization using single fluorescent dye

Patent ·
OSTI ID:869894
 [1];  [2]
  1. Los Alamos, NM
  2. Irvine, CA
+ Show Author Affiliations
Chromosomes are characterized by fluorescent emissions from a single fluorescent dye that is excited over two different wavelengths. A mixture containing chromosomes is stained with a single dye selected from the group consisting of TOTO and YOYO and the stained chromosomes are placed in a flow cytometer. The fluorescent dye is excited sequentially by a first light having a wavelength in the ultraviolet range to excite the TOTO or YOYO to fluoresce at a first intensity and by a second light having a wavelength effective to excite the TOTO or YOYO dye to fluoresce at a second intensity. Specific chromosomes may be identified and sorted by intensity relationships between the first and second fluorescence emissions.
Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM
DOE Contract Number:
W-7405-ENG-36
Assignee:
Regents of University of California, Office of Technology (Alameda, CA)
Patent Number(s):
US 5418169
OSTI ID:
869894
Country of Publication:
United States
Language:
English

References (2)

High-speed chromosome sorting journal October 1987
Fluorometric Assay Using Dimeric Dyes for Double- and Single-Stranded DNA and RNA with Picogram Sensitivity journal January 1993

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