(Comparative) mutagenesis of human cells in vivo and in vitro
We have combined Fischer and Lerman's denaturing gradient gel electrophoresis, high fidelity DNA amplification, and quantitative mutational measurements in a 104 bp sequence within the third exon of the hprt gene in cultured human TK6 cells in order to observe predominant point mutations. We have now characterized the mutations arising spontaneously or after treatment with ICR-191, MNNG, benzo({alpha})pyrene diol epoxide, ultraviolet light, hyperbaric oxygen, and hydrogen peroxide.
- Research Organization:
- Massachusetts Inst. of Tech., Cambridge, MA (United States)
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-86ER60448
- OSTI ID:
- 7235709
- Report Number(s):
- DOE/ER/60448-T4; ON: DE92016842
- Country of Publication:
- United States
- Language:
- English
Similar Records
[Comparative] mutagenesis of human cells in vivo and in vitro. Progress report, 1989
Comparative mutagenesis in human cells in vitro and in vivo: First progress report for the period 15 July 1986-30 June 1987
Determination of point mutational spectra of benzo[a]pyrene-diol epoxide in human cells
Technical Report
·
Sat Dec 30 23:00:00 EST 1989
·
OSTI ID:10158113
Comparative mutagenesis in human cells in vitro and in vivo: First progress report for the period 15 July 1986-30 June 1987
Technical Report
·
Mon Jun 01 00:00:00 EDT 1987
·
OSTI ID:5603649
Determination of point mutational spectra of benzo[a]pyrene-diol epoxide in human cells
Journal Article
·
Sat Oct 31 23:00:00 EST 1992
· Environmental Health Perspectives; (United States)
·
OSTI ID:6848508
Related Subjects
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BENZOPYRENE
BIOASSAY
CELL CULTURES
CONDENSED AROMATICS
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
GENE MUTATIONS
HYDROCARBONS
MAMMALS
MAN
MUTATION FREQUENCY
MUTATIONS
ORGANIC COMPOUNDS
PRIMATES
PROGRESS REPORT
RADIATIONS
ULTRAVIOLET RADIATION
VERTEBRATES
Cells
& Tissue Culture
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BENZOPYRENE
BIOASSAY
CELL CULTURES
CONDENSED AROMATICS
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
GENE MUTATIONS
HYDROCARBONS
MAMMALS
MAN
MUTATION FREQUENCY
MUTATIONS
ORGANIC COMPOUNDS
PRIMATES
PROGRESS REPORT
RADIATIONS
ULTRAVIOLET RADIATION
VERTEBRATES