[Comparative] mutagenesis of human cells in vivo and in vitro. Progress report, 1989
We have combined Fischer and Lerman`s denaturing gradient gel electrophoresis, high fidelity DNA amplification, and quantitative mutational measurements in a 104 bp sequence within the third exon of the hprt gene in cultured human TK6 cells in order to observe predominant point mutations. We have now characterized the mutations arising spontaneously or after treatment with ICR-191, MNNG, benzo({alpha})pyrene diol epoxide, ultraviolet light, hyperbaric oxygen, and hydrogen peroxide.
- Research Organization:
- Massachusetts Inst. of Tech., Cambridge, MA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-86ER60448
- OSTI ID:
- 10158113
- Report Number(s):
- DOE/ER/60448--T4; ON: DE92016842
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120
560300
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BENZOPYRENE
BIOASSAY
CELL CULTURES
CHEMICALS METABOLISM AND TOXICOLOGY
ELECTROPHORESIS
GENE MUTATIONS
MAN
MUTATION FREQUENCY
PROGRESS REPORT
RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
ULTRAVIOLET RADIATION
560300
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BENZOPYRENE
BIOASSAY
CELL CULTURES
CHEMICALS METABOLISM AND TOXICOLOGY
ELECTROPHORESIS
GENE MUTATIONS
MAN
MUTATION FREQUENCY
PROGRESS REPORT
RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
ULTRAVIOLET RADIATION