Combined carcinogen bioassay utilizing both the lung adenoma and skin papilloma protocols
To test the feasibility of employing a combined lung adenoma/skin papilloma assay for broader detection of chemical carcinogenesis than that realized with either bioassay done separately, four strains of mice, SENCAR, BALB/c, A/J, and ICR-Swiss, were administered carcinogens either by the oral or intraperitoneal (IP) routes. The carcinogens administered were ethyl carbamate (EC), benzo(a)pyrene (B(a)P), N-(4-(5-nitro-2-furyl)thiazolyl)formamide (FANFT), and acrylamide (ACR). Starting 2 weeks later, 1 to 5 ..mu..g (depending on strain) of 12-O-tetradecanoylphorbol-13-acetate (TPA) in 0.2 mL acetone/mouse was applied three times weekly to the shaved back for 20 weeks. All strains displayed increases in the yield of lung adenomas in response to EC at 32 weeks. B(a)P increased lung adenomas in only the SENCAR and A/J strain. Only the SENCAR and ICR-swiss mice gave positive responses in the skin. In the SENCAR mice, positive response was seen with all four chemicals, however, FANFT gave an inconsistent response. The ICR-Swiss mice responded with an increased skin papilloma yield only to EC. In a separate experiment involving only SENCAR mice, animals were treated with a single oral dose of diethylnitrosamine (DEN) followed by triweekly application of 1.0 ..mu..g TPA. This treatment resulted in 51/57 animals developing lung adenomas vs. 5/57 in the control animals. No treatment-related skin tumors resulted with DEN. Histopathologically confirmed lesions indicate that the spectrum of chemicals detected in the SENCAR mouse may be broadened using a combined bioassay that examines both lung and skin responses.
- Research Organization:
- Environmental Protection Agency, Cincinnati, OH
- OSTI ID:
- 7232568
- Journal Information:
- Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 68; ISSN EVHPA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACRYLAMIDE
ADENOMAS
AMIDES
ANIMALS
AROMATICS
BENZOPYRENE
BIOASSAY
BIOLOGICAL EFFECTS
BODY
CARBAMATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CARCINOGENESIS
CARCINOGENS
CONDENSED AROMATICS
DISEASES
ESTERS
HISTOLOGY
HYDROCARBONS
INFLAMMATION
LUNGS
MAMMALS
MICE
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PATHOLOGICAL CHANGES
PHORBOL ESTERS
PROMOTERS
RESPIRATORY SYSTEM
RODENTS
SKIN
SPECIES DIVERSITY
SYMPTOMS
TUMOR PROMOTERS
VERTEBRATES