Characterization of the gene encoding human cone transducin [alpha]-subunit (GNAT2)
- Purdue Univ., West Lafayette, IN (United States)
- Indiana Univ., Indianapolis, IN (United States)
The human cone transducin [alpha]-subunit (GNAT2) gene has been completely characterized. The human GNAT2 transcription unit is 9967-bp in length and consists of eight exons with seven introns. The eight exons are identical to the reported cDNA sequence. Northern blot analysis of RNA from human retinas and a retinoblastoma cell line, WERI-RB1, reveals a 1.7-kb transcript for GNAT2. Multiple transcription initiation sites were mapped for human retina and WERI-RB1 RNA by primer extension and S1 nuclease protection assays. This gene has seven initiation sites spanning 31 bp. The sequence upstream of the GNAT2 gene shows a TATA box consensus sequence at -29, a CCAAT box consensus sequence at -58 (reverse orientations), and a sequence (CCATAT) similar to the CCAAT box consensus at -76. The GNAT2 upstream sequence shows no significant identity with the upstream region of the human rod transducin [alpha]-subunit gene (GNAT1) or with the upstream regions of the color visual pigment genes, indicating that the expression of GNAT2 may be regulated differently than these other rod- and cone-specific proteins. 35 refs., 6 figs., 2 tabs.
- OSTI ID:
- 7225885
- Journal Information:
- Genomics; (United States), Vol. 17:2; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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